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KMID : 0380620080400030354
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Korean Journal of Food Science and Technology
2008 Volume.40 No. 3 p.354 ~ p.359
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Safety/Toxicology : A Survey of Zearalenone in Beans Using High Performance Liquid Chromatography-Fluorescence Detector (HPLC-FLD) and Ultra Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
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Jang Mi-Ran
Lee Chang-Hee
Lee Hyo-Jung
Kim Ji-Yeon
Son Sang-Hyuk
Shin Choon-Shik
Kim So-Hee
Kim Dae-Byoung
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Abstract
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A survey for zearalenone contamination was conducted on 27 soy bean samples, 27 red bean samples, 16 black bean samples, 19 seoritae samples, 14 seomoktae samples, for a total of 127 commercial Korean samples. Zearalenone was quantified by the immunoaffinity column clean-up method with high performance liquid chromatography-fluorescence detection (HPLC-FLD), and was confirmed by liquid chromatography tandem mass spectrometry(LC-MS/MS). The limits of detection and quantification were 2.0 μg/kg and 6.0 μg/kg, respectively. The recovery in the beans ranged from 82.2 to 98.4%. According to HPLC-FLD, zearalenone was detected in 13 samples (10.2% incidence), including 1 soybean and 12 red bean samples. The zearalenone contamination levels were in the range of 8.01~38.98 μg/kg. Finally, LC-MS/MS analysis was conducted in the contaminated samples to verify the results of HPLC-FLD. The LC-MS/MS results confirmed the presence of zearalenone in all 13 samples. The contamination level was lower than that of EU, which is below 100 μg/kg for raw grains.
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KEYWORD
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mycotoxin, zearalenone, immunoaffinity column, beans
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